Given that HDAC1 and HDAC3 targeted by MS-275 mainly account for the deacetylation of histone subunits 3 (H3), especially on histone H3 lysine9 (H3K9), H3 lysine 14 (H3K14), and H3 lysine 27 (H3K27) [12–14], we sought to clarify if the acetylation of H3K9, H3K14, and H3K27 (H3K9ac, H3K14ac, and H3K27ac) involve in changed gene profile driving differentiation form cancer cells to neuron-like cells. This evidence concerns the gene HDAC3 and cancer.