Further, primary MIBC patients expressing higher levels of MAP4K4 had significantly higher metastatic potential compared to the ones expressing MAP4K4 at lower levels (Supplementary Fig. 11c, d) in two cohorts of the TCGA bladder cancer studies8,9, making MAP4K4 is a prominent FRA1 and FLI1 regulated gene critical for metastasis of bladder cancer. This evidence concerns the gene FLI1 and urinary bladder cancer.