On the other hand, transcription factors p63, a tumor suppressor with differential variant expression and involved in bladder tumorigenesis46, GRHL2, a transcription factor supporting the expression of epithelial genes and deregulated in various cancers47, and the PPARg, a nuclear receptor highly mutated in bladder cancer and implicated in urothelial differentiation48 were significantly enriched at NMIBC-specific regulatory elements and significantly overexpressed in NMIBC lines and NMIBC-class patients (Fig. 3c, d and Supplementary Fig. 6). This evidence concerns the gene GRHL2 and urinary bladder cancer.