The binding affinity between the transcription factor EGR1 and Gαi2 promoter was also significantly increased in primary (pHCC1/ pHCC-2/ pHCC-3) and HepG2 HCC cells, when compared to the low binding between the two in primary human hepatocytes and immortalized HL-7702 cells (Fig. 7I). This evidence concerns the gene EGR1 and hepatocellular carcinoma.