To determine whether GPR40 regulates the RhoA/ROCK in obese asthma in our study, we showed that there was a significant increased expression of GTP-RhoA and ROCK1 in the lung tissues of obese asthmatic mice and OA-treated HASM cells, whereas, targeting GPR40 with DC260126 markedly suppressed GTP-RhoA and ROCK1 expression both in vivo and in vitro. This evidence concerns the gene ROCK1 and asthma.