TRDMT1 and metabolic disease: By using a high-fat diet-induced metabolic disorder mouse model, we also identified that the level of tsRNA m5C was correlated with the level of N2-Methylguanosine (m2G) on tsRNA, and deletion of the m5C methyltransferase Dnmt2 decreased the level of m5C on tsRNA and coordinately downregulated the level of m2G on tsRNA [45].