Because FXR regulates BA homeostasis, its knockout (KO) in mice causes BA synthesis dysregulation and leads to the development of non-alcoholic fatty liver (5 months old), which progresses into NASH (10 months old) and HCC (15 months old) even when FXR KO mice consume a healthy diet [11, 12]. This evidence concerns the gene NR1H4 and hepatocellular carcinoma.