Moreover, we correlated the functional expression of FBXO31 with ZNF529-AS1 by predicting that FBXO31, as a member of the forkhead box protein family, was reported to play an important role in immune infiltration of tumours[36], such as in hepatocellular carcinoma, lung cancer, breast cancer and colorectal cancer[37–40], in which forkhead box proteins play an important role in the function of immune infiltration. Here, ZNF529 is linked to colorectal cancer.