Our data on TILs indicate 1) the increased frequency of WT1-specific CTLs in CD8+ T cells, 2) the obvious production of WT1-specific IFN-γ by CD4+ T cells, and 3) the strengthened tumor-infiltrating capability of WT1-specific CTLs primed in the intestine [16], all of which afford flow cytometric evidence about the antitumor activity of combining B. longum 420 for WT1-specific CD8+ T cell activation and B. longum 2656 for CD4+ T cell help. This evidence concerns the gene CD8A and neoplasm.