To further scale down the candidate genes and identify credible key genes during osteosarcoma development, multiple survival analysis was then in succession performed to evaluate the association between each of the 22 genes and osteosarcoma patients survival, and the result revealed three genes: STC2, FGF2 and PRKCSH, they were all supported to be associated with both patients overall and recurrence free survival. Here, STC2 is linked to osteosarcoma.