To further scale down the candidate genes and identify the promising responsible key gene during osteosarcoma development, KM survival analysis was performed using TARGET osteosarcoma transcriptome data to analyze the overall survival of the 22 extracellular matrix regulation related genes, and the result indicated that 3/22 genes were associated with both osteosarcoma overall survival and disease-free survival, namely FGF2 (Fig. 4A and D), PRKCSH (Fig. 4B and E) and STC2 (Fig. 4C and F). This evidence concerns the gene STC2 and osteosarcoma.