To determine the contribution of the PIP4K2C phosphoinositide kinase in induction of cell death, melanoma A375 cells were treated with siRNA against the PIP4K2C gene (siPIP4K2C) and then subsequently cultured in the presence of either vehicle or 0.05 μM WX8 to selectively inhibit PIKFYVE. Here, PIP4K2C is linked to melanoma.