PIKFYVE and cancer: The ability of WX8 to selectively inhibit PIKFYVE at low concentrations and both PIKFYVE and PIP4K2C at higher concentrations revealed that the distinction between PIKFYVE-dependent (autophagy-dependent) cancer cells and nonmalignant cells resulted from a deficiency in PIP5K1C that prevented nonmalignant cells from producing PtdIns(4,5)P2 in the absence of PIKFYVE.