To assess the impact of BPLF1 and its DUB activity on EBV infection, different combinations of cGAS + STING, TBK1, BPLF1 and C61A were expressed in HEK293 cells, which were subsequently infected with freshly prepared GFP-marked EBV M81 virus 24 hours after transfection. This evidence concerns the gene TBK1 and Epstein-Barr virus infection.