Extending our prior immunoblotting work in cell-based assays6,7,10–12, screening of SW48, CaCo2, HT29, LoVo and SW620 human colon cancer cell lines revealed moderate to high constitutive expression of ACE2, BRD2 and BRD4 proteins compared with CCD841 normal colonic epithelial cells (Supplementary Fig. 1). This evidence concerns the gene BRD4 and malignant colon neoplasm.