Thus, we employed knockout cell lines of various TLRs such as ΔTLR1, ΔTLR2/4, ΔTLR4, ΔTLR6, ΔTRIF, and ΔMYD88/TRIF decipher the innate immune receptor involved in producing anti-TB cytokines, including TNF, IL6, and IL12 using ELISA. The gene discussed is IL6; the disease is tuberculosis.