ACHE and cardiovascular disorder: Kawano et al. investigated the distribution of autonomic nerves in autopsied hearts of humans without cardiovascular disease, using immunolabelling techniques for a cholinergic modulator (acetylcholinesterase, AChE) and an adrenergic marker (tyrosine hydroxylase, TH) (Kawano et al., 2003; Kawashima, 2005).