CNR1 and alkaline phosphatase measurement: The results showed that 8 key active compounds may play pivotal roles in both anti-inflammatory and inhibition of intracellular Ca2+ overload effects in AP treatment by acting primarily on TNF, IL-1β, PPARγ, PPARα, PTGS2, NCOA1, CNR1, and ESR1 and involving in neuroactive ligand-receptor interaction, estrogen, MAPK, and calcium signaling pathway.