The results showed that anti-CTLA-4 initiated inhibition of Treg cells to expand the CD8/Treg ratio, and anti-PD-1/PD-L1 mainly increased the proportion of CD8+ TIL, but higher responses were obtained only with the involvement of RT, which diversified the T-cell receptor (TCR) of unirradiated tumor TIL (137). The gene discussed is CD274; the disease is neoplasm.