TerraFlow identified a number of phenotypes, not revealed by tSNE analysis, that differed between HCC and PDA (Figure 2E), variously highlighting the elevation of ICOS, TIGIT, CD69, and PD1 in distinguishing PDA from HCC (Supplementary Figures 2A–E) using machine learning and recursive feature analysis (14). This evidence concerns the gene PDCD1 and hepatocellular carcinoma.