Immunohistochemical analysis of breast cancer tissues showed that 41% (59/144) expressed high SP (TS > 5), 36.8% (53/144) expressed high NK1R (TS > 1), 42.2% (61/144) expressed high Ki-67 (expression > 22%), and 38.2% (55/133) expressed high PKM2 (TS > 4). The gene discussed is PKM; the disease is breast cancer.