When classifying serum Klotho into quartiles, we found that Klotho was not significantly associated with anemia at Q3 (840.8- 935.5 pg/mL) (p > 0.05), while at Q1 (496.7 - 613.9 pg/mL) and Q2 (690.9-763.4 pg/mL) (p < 0.05), the association was significant. The gene discussed is KL; the disease is anemia.