Since HIF-1α is implicated in neuroprotection and inflammation inhibition, a recent study has demonstrated that roxadustat administration, an anti-anemia medication, ameliorated migraine-like behaviors and inhibited central pain sensitization in nitroglycerin-injected mice, which was mainly mediated by HIF-1α/NF-κB/inflammation pathway [99]. This evidence concerns the gene NFKB1 and anemia (phenotype).