SHH and colorectal cancer: The results were as follows: (1) 1′-O-methyl-averantin was cytotoxic to CSC221, CaCo2, DLD1, and HCT116 cells; (2) 1′-O-methyl-averantin and the crude extract suppressed spheroid formation by colorectal cancer cells; (3) 1′-O-methyl-averantin and the crude extract downregulated expression of cancer stem cell markers; (4) 1′-O-methyl-averantin and the crude extract inhibited the SHH signaling pathway was markedly inhibited with 1′-O-methyl-averantin in CSCs cell; and (5) 1′-O-methyl-averantin and the crude extract inhibited Notch signaling at the transcriptional level.