DLD1 has mutant KRAS, BRAF, PIK3CA, TP53, and APC and MSI; Caco2 has wild-type KRAS, BRAF, PIK3CA, TP53, APC and MSS; HCT116 has KRAS mutant; CSC221 is human colorectal adenocarcinoma-enriched cancer stem cell50,51. This evidence concerns the gene TP53 and colorectal adenocarcinoma.