To define and cross-validate the transcriptional signature of the circulating CD431B11+ T cell subsets, we performed bulk mRNA sequencing on fluorescence-activated cell-sorted CD431B11− and CD431B11+ CD8+ and CD431B11− and CD431B11+ CD4+ T cells from MC-38 tumor-challenged mice that were treated with PDOX. This evidence concerns the gene CD8A and neoplasm.