The results revealed that the expression of HSP90AA1 (Fig 7A), PPIA (Fig 7B), SQSTM1 (Fig 7C), and USP21 (Fig 7D) was significantly increased in liver cancer tissues compared with normal liver tissues (p < 0.05, S2 Table), which was consistent with the results of the bioinformatics analysis. The gene discussed is HSP90AA1; the disease is liver cancer.