mTORC1 activation, as assessed by phospho-S6 staining, was sporadically present in cystic and noncystic epithelia, suggesting that TFEB translocation did not directly parallel the activity of mTORC1 and suggesting that increased mTORC1 activity may not be a primary driving force in renal cyst development when contrasted with the observed ubiquitous nature of TFEB nuclear translocation. The gene discussed is TFEB; the disease is cystic kidney disease.