As observed with the other murine models of renal cystic disease examined, costaining of Tfeb with the nuclear marker Hoechst 33342 as assessed by immunofluorescence (see Supplemental Fig. S5) revealed upregulation and significant nuclear colocalization (Supplemental Fig. S5, A–C) compared with wild-type cells (Supplemental Fig. S5, D–F). Here, TFEB is linked to cystic kidney disease.