In the current study, by in vitro study, we confirmed that over expression of TUG1 could significantly reduce the Collagen IV and fibronectin expression in HG-treated HK2 cells; Meanwhile, by AAV-TUG1 lentivirus vector delivery, we also confirm that exogenous TUG1 administration also significantly improve the renal function and ameliorate the interstitial fibrosis in DN mice. The gene discussed is FN1; the disease is liver dysplastic nodule.