Furthermore, ISM042-2-048 also demonstrated good anti-proliferation activity in an HCC cell line Huh7 with high expression levels of CDK20 (IC50 = 208.7 ± 3.3 nM) whereas less effect was seen in the counter screen cell line HEK293 (IC50 = 1706.7 ± 670.0 nM). Here, CDK20 is linked to hepatocellular carcinoma.