To establish whether NQO1-induced CKS1 regulates cancer cell cycle progression at G2/M phase, we transfected NQO1-overexpressing cancer cells with siCont and pCont or siCKS1B and pCont, and NQO1-deficient cancer cells with pCont and siCont or pCKS1B and siCont. Here, NQO1 is linked to cancer.