To verify whether the alteration of gluconeogenesis contributes to an increase in blood glucose in Ptp4a1-/- mice fed an HF diet compared to controls, we performed the pyruvate tolerance test (PTT) and glycerol tolerance test (GlyTT) in WT and Ptp4a1-/- mice fed an HF diet. Here, PTP4A1 is linked to hydrops fetalis.