To evaluate whether PTP4A1 affects the regulation of obese-mediated metabolic diseases such as type 2 diabetes and NAFLD, we generated Ptp4a1-/- mice by CRISPR/Cas9 system (Figure S2) and placed Ptp4a1-/- mice and WT littermates on an HF diet for 12 weeks. Here, PTP4A1 is linked to metabolic disease.