To explore the effects of hyperactive autophagy in DG neurons on neuronal BDNF, AHN, and depression-like behaviors, we knocked down Atg5 expression in the hippocampal DG neurons of CORT mice using AAV-hSyn-miR30-shRNA (Atg5) (Figure S2C and S2D). The gene discussed is RIC8B; the disease is major depressive disorder.