The younger patients had pNF-H levels above the cut-off for adult-onset ALS patients (cut-off: 529 pg/mL; Patient 2: 1748, Patient 3: 785 pg/mL), while NF-L levels were below the cut-off of 45 pg/mL (Patient 2: 37.4 pg/mL, Patient 3: 34.1 pg/mL). This evidence concerns the gene NEFL and amyotrophic lateral sclerosis.