The activation of myeloid cells and NK cells in addition to the activation of cytotoxic T cells was reported after TLR7 agonist stimulation (37, 38), and the increased invasion of myeloid cells was reported in EMT6 model mice (39), suggesting immune cells other than CD8+ T cells contribute to the anti-tumor effects of DSP-0509 in the EMT6 model. This evidence concerns the gene DSP and neoplasm.