First, 556 differentially expressed genes of tuberculosis were screened out, then the PPI regulatory network was constructed and the key nodes of the PPI regulatory network through were screened out by the combination of three algorithms (DC, BC and CC), and finally we found six key genes (AKT1, TP53, EGF, ARF1, CD274 and PRKCZ). The gene discussed is EGF; the disease is tuberculosis.