PRF1 and colonic neoplasm: Its signaling significantly hindered the killing activity of Vγ9Vδ2 T cells against colon cancer cells and reduced the expression of perforin and granzyme B cells in an ERK1/2-dependent manner (92); because γδ T cells express high levels of Tim-3 in peripheral blood and colon cancer tissue, Tim-3 blockade by neutralizing antibody restores their cytotoxicity towards colon cancer cells.