Methylation-related mechanisms are complex, and the specific mechanisms, such as DNA methylation or histone methylation, responsible for DNA methylation of TYROBP, BIRC5, BUB1B, CENPF, and MELK are still needed to be further investigated through methylation-specific PCR detection of clinical ccRCC samples. This evidence concerns the gene MELK and nonpapillary renal cell carcinoma.