PRF1 and colonic neoplasm: Therefore, blocking TIM-3 can increase the killing effects of Vγ9VδT cells on colon cancer cells by activating the ERK1/2 pathway and upregulating the expression of perforin and granzyme B. B7-H3 also inhibits the cytotoxicity of Vδ2T cells by downregulating the expression of perforin and granzyme B [168], which can be reversed by using B7-H3 blockers.