On performing regression analysis between ΔCT values (which are inversely proportional to the amount of mRNA in the sample) of selected markers and other clinical and laboratory parameters in the post-OCP treated PCOS group, it was observed that fasting insulin (β = − 0.298; p = 0.025) and 2 h insulin (β = − 0.849; p = 0.00) independently predicted ICAM-1 expression. This evidence concerns the gene ICAM1 and polycystic ovary syndrome.