Immunohistochemistry for ACTH, growth hormone, thyroid-stimulating hormone, luteinizing hormone, follicle-stimulating hormone, prolactin, Ki67, and P53 was performed to confirm the diagnosis of ACTH-producing adenoma (Fig. 3B) and the samples were negative for markers of the other pathological type (Fig. 3C-I). This evidence concerns the gene GH1 and adenoma.