To explore why ROBO1 is enriched in liver metastases and how SLIT2-ROBO1 manipulates the selection of tumour cells in coadaptation, we next used a cell mixture composed of 50% Panc02Robo1-FL and 50% Panc02CTRL cells (PG0) in an intrasplenic injection mouse model. Here, ROBO1 is linked to neoplasm.