To study the role of the SLIT2-ROBO1 interaction in the coadaptation of cells in PDAC liver metastasis, tumour cells expressing full-length ROBO1 protein (Kpc1199Robo1-FL or Panc02Robo1-FL) were prepared (Supplementary Fig. 5a, b). Here, ROBO1 is linked to neoplasm.