For instance, Yu-Chen Ye et al. found that the dominating TAMs in orthotopic HCC in conditional disruption of the recombination signal binding protein Jκ (RBPj cKO) mice manifested properties of Kupffer cells (KCs), thus suggesting that TAMs (KC-like TAMs, F4/80+CD11bloLy6clo/− TAMs) in HCC in situ were more likely to originate from embryonic hematopoiesis–generated KCs and bone marrow (BM) monocyte-derived KCs, which are considered as the self-renewing tissue-resident macrophages [30–32]. The gene discussed is CALCA; the disease is hepatocellular carcinoma.