ASAP1 and neoplasm: To understand whether the mechanism by which ASAP1 enhances drug resistance in tumor cells is related to EGFR, We detected the expression of the EGFR-MAPK pathway in SGC-7901 cells after overexpressing ASAP1 and found that EGFR protein expression, phosphorylated MAPK, and total MAPK expression increased, whereas there was an opposite trend in ASAP1+/−-SGC-7901 cells (Fig. 8B) In addition, we investigated the effect of ASAP1 on the subcellular localization of EGFR using an IF assay.