KCNJ16 and Hypokalemia: The double Nedd4-2/Kir4.1 KO mice, however, did not develop hypokalemia, as did Kir4.1/Kir5.1 KO mice, probably because the deletion of Nedd4-2 resulted in less NCC degradation and preservation of the electroneutral Na+ reabsorption in DCT (73) and lower Na+ load into principal cells.