Phenotypes observed in double KO of NCC and pendrin have led to the suggestion of pharmacologic inhibition of pendrin and NCC to provide a novel and strong diuretic regimen for patients with fluid overload, including those with congestive heart failure, nephrotic syndrome, or renal failure (35), which would induce huge Na+ and water excretion without significant K+ loss. This evidence concerns the gene SLC12A3 and congestive heart failure.