Given that mutations in the Ncc gene were identified as the cause of Gitelman syndrome (19), it was expected that the knockout (KO) of this gene (Ncc-KO) would cause the changes observed in patients with this syndrome, namely, decreased extracellular volume with mild arterial hypotension, hypokalemia, metabolic alkalosis, hypocalciuria, and hypomagnesemia. This evidence concerns the gene SLC12A3 and Hypokalemia.