SART3 and amyotrophic lateral sclerosis: To validate this hypothesis, we searched the publicly available data sets and found SART3 knockdown combined with poly-A RNA-seq data using Hep G2 cells.22 In fact, aberrant alternative splicing such as intron retention is frequently observed in post-mortem samples of ALS patients.45,46 We therefore compared the SART3 knockdown with the control to quantify intron retention events using IRfinder.23 In general, we observed lower intron retention in the SART3 knockdown cells (Fig. 3C and Supplementary Table 2).