Utilizing flow cytometry, ECs stimulated with conditioned media (CMs) from uninfected or oHSV (rHSVQ)-infected glioma cells in the presence/absence of neutralizing HMGB1 antibody (oHSV-infected/uninfected CM ± αHMGB1) were assessed for changes in expression of cell surface markers, indicative of EC activation. This evidence concerns the gene HMGB1 and central nervous system cancer.