Therefore, to develop an optimal combination of pharmacological and physical activity treatments for the amelioration of diabetic vascular dysfunction, in this work we examined the minimal and optimal doses of two types of exercise training and UA consumption on vascular aging‐related variables in old male Wistar rats with high‐fat diet and or low‐dose streptozotocin‐induced type 2 diabetes (HFD/STZ‐induced T2D) with regard to the SIRT1–eNOS axis and OS biomarkers. Here, NOS3 is linked to type 2 diabetes mellitus.