ALT and AST are known an important biomarkers related to hepatocytes function, and the increased plasma concentration of aforementioned enzymes implied occurrence of Cholestasis, imbalance of lipid and carbohydrate metabolism, hepatotoxicity and hepatocytes injury, which are associated with NAFLD and hyperlipidemia (Chang et al., 2013; Khodadoostan et al., 2016; Taliercio et al., 2013). The gene discussed is GPT; the disease is cholestasis.