While no MV1 organoid homogenate inoculated mice (MV1-OH) showed clear clinical signs of prion disease out to 700 dpi, two were found dead (296 and 302 dpi) and one was euthanized for other reasons (609 dpi) and their brain tissue found to be RT-QuIC positive, with PrP deposition detected by western blot and, in the one case where available, positive for immunohistochemical (IHC) hallmarks of prion disease (Fig. 1b–d, Fig. 2, Table 2). This evidence concerns the gene PRNP and prion disease.