In the current study, we constructed a prodromal PD model by injecting adeno-associated viruses (AAVs) containing a human mutated A53T form of the α-synuclein gene into the left cervical vagus nerve, which exhibited gastrointestinal AutD and disruption of neural integrity 3 months prior to the motor symptoms and loss of dopaminergic neurons. This evidence concerns the gene SNCA and Parkinson disease.