Eleven biomarkers were significantly lower in GDM-M group [CLUL1 (Clusterin-like protein 1), VCAN (Versican core protein), FCRL1 (Fc receptor-like protein 1), RNASE3 (Eosinophil cationic protein), APLP1 (Amyloid-like protein 1), REG4 (Regenerating islet-derived protein 4), CANT1 (Soluble calcium-activated nucleotidase 1), FKBP4 (Peptidyl-prolyl cis-trans isomerase FKBP4), GHRL (Appetite-regulating hormone), MEP1B (Meprin A subunit beta), CD79B (B-cell antigen receptor complex-associated protein beta chain)], and CLMP (CXADR-like membrane protein) was significantly higher in GDM-M group. This evidence concerns the gene REG4 and gestational diabetes.