To produce a model of EMT that is mediated by levels of Snail overexpression comparable to those that arise spontaneously in cancer cell lines, a single copy expression cassette of human SNAI gene (Snail) that was doxycycline-inducible was engineered into a defined, open, genomic region in DLD-1 cells using frt recombination (Sup. This evidence concerns the gene SNAI1 and cancer.