During mid-term calcification, AR positive nodules increased prominently in the OM only and OM cotreated with TGF-β1 groups compared to the control (Figure 2D, 2E), while the TGF-β1 only group exhibited only a trend of increasing calcification compared to the control without significant differences during mid-term calcification (Figure 2F). The gene discussed is AR; the disease is ocular melanoma.